End with an objective statement where you briefly sum-up what you set out to do in this set of experiments. Include a brief hypothesis.
M&M 250 words: be succinct, concise, and brief while allowing an informed reader to be able to replicate your work. Do not show calculations for prelabs.
Results: show images of your results that you have already taken
CAPTIONS: make sure you have good captions (captions don’t count against the word limit)
Transformation plates images - Mention how many colonies you got. Flask images, purple pellet images (include the weight of your pellet)
Images of Elution 1 and 2 in tubes, Nanodrop screen shots (of 280nm reading for Elution 1 only) - Include yields from your Nanodrop spectrophotometry for your protein amounts.
Show brief calculations for Beer’s law calculations. A=Ebc
Protein Gel images, Image of Ladder from web as reference (molecular weight marker used – get from website of company)
Discusssion
Analyze your results, address sources of error, and answer any questions from the handout
Q: Why do we use lysozyme? Why do we use Benzonase/Cyanase?
Q: Briefly, address how the HIS tag system works
Q: Explain what is in the sample after each step: Sample 1, 2, 3,4 ,5, and 6
Q: What is different about the Wash vs. the Elution buffer?
Q: State the size of your protein from the gel and compare it to the size you determined in the protein purification lab
Q: address the purity of your sample
Conclusions
Recap what was done
State key finding(s)
Address future directions/applications of this work to VDS research
MARIA B.
ANNA B.
TAYLOR C.
SARA C.
SAMANTHA D.
MICHELLE D.
ANDREA F.
JAMES F.
JOHN G.
CIDIA G.
XENIA G.
SANIYA H.
SAUL H.
ARIANA L.
MONICA L.
ANDREI L.
LAUREN M.
SRICHARAN N.
DAREN N.
ASHLY O.
CARLOS O.
TRAM P.
NEUSHA R.
MEGAN S.
JUSTIN S.
David T.
CHRISTOPHER T.
CAROLYN T.
TYLER T.
ALBERTO T.
NATANIA T.
LUIS V.
JAIRO V.
NICOLE W. SP14
AVERY W.
KEENAN W.
SANJNA Z.
Online Report: use your page on the WikiSpaces (under Protein Labs)
Lab Report:
This report will encompass the last three (3) labs: protein expression, purification, and characterization.
1,000 words total –
Since this is online – you must be efficient with your words! Make them count!
Title: think of something semi-creative
Intro words: this should have some background information on technique of protein production and purification.
There is a good article here: Nat Methods. 2008 Feb;5(2):135-46. Protein production and purification.
Also a good website: http://www.embl.de/pepcore/pepcore_services/index.html
End with an objective statement where you briefly sum-up what you set out to do in this set of experiments. Include a brief hypothesis.
M&M 250 words: be succinct, concise, and brief while allowing an informed reader to be able to replicate your work. Do not show calculations for prelabs.
Results: show images of your results that you have already taken
CAPTIONS: make sure you have good captions (captions don’t count against the word limit)
Transformation plates images - Mention how many colonies you got. Flask images, purple pellet images (include the weight of your pellet)
Images of Elution 1 and 2 in tubes, Nanodrop screen shots (of 280nm reading for Elution 1 only) - Include yields from your Nanodrop spectrophotometry for your protein amounts.
Show brief calculations for Beer’s law calculations. A=Ebc
Protein Gel images, Image of Ladder from web as reference (molecular weight marker used – get from website of company)
Discusssion
Analyze your results, address sources of error, and answer any questions from the handout
Q: Why do we use lysozyme? Why do we use Benzonase/Cyanase?
Q: Briefly, address how the HIS tag system works
Q: Explain what is in the sample after each step: Sample 1, 2, 3,4 ,5, and 6
Q: What is different about the Wash vs. the Elution buffer?
Q: State the size of your protein from the gel and compare it to the size you determined in the protein purification lab
Q: address the purity of your sample
Conclusions
Recap what was done
State key finding(s)
Address future directions/applications of this work to VDS research
References (2-3)
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